Phosphorylation of histone H3 threonine 11
by the Tda1 kinase under nutritional stress
requires the AMPK and CK2 kinases

Dr Jerry L Workman
Stowers Institute for Medical Research
Kansas City, USA

25th November, 2019

Seminar Hall, 3rd Floor
CDFD, Uppal


To understand upsteam signaling of H3pT11, we dissected kinases involved in this process. We found that H3pT11 requires both AMPK and CK2 kinases. However, these kinases do not directly phosphorylate H3T11 but instead directly phosphorylate the Tda1 kinase. Tda1 is a yeast AMPK interacting kinase that phosphorylates H3T11 in vitro and in vivo. Phosphorylation of Tda1 is required for its proper histone kinase activity and its protein stability. The Tda1 kinase thus integrates signals of multiple upstream kinases to regulate H3T11 phosphorylation upon nutrient stress.